Volume 17, Issue 1 pp. 54-58

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A Disposable Biosensor for Organophosphorus Nerve Agents Based on Carbon Nanotubes Modified Thick Film Strip Electrode

Kanchan A. Joshi,

Kanchan A. Joshi

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Jason Tang,

Jason Tang

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Robert Haddon,

Robert Haddon

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

Department of Chemistry, University of California, Riverside, CA 92521, USA

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Joseph Wang,

Joseph Wang

Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA

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Wilfred Chen,

Wilfred Chen

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Ashok Mulchandani,

Ashok Mulchandani

[email protected]

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Kanchan A. Joshi,

Kanchan A. Joshi

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Jason Tang,

Jason Tang

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Robert Haddon,

Robert Haddon

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

Department of Chemistry, University of California, Riverside, CA 92521, USA

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Joseph Wang,

Joseph Wang

Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA

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Wilfred Chen,

Wilfred Chen

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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Ashok Mulchandani,

Ashok Mulchandani

[email protected]

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

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First published: 18 January 2005

https://doi-org.bibliotheek.ehb.be/10.1002/elan.200403118

Citations: 199

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Abstract

A disposable biosensor based on acetylcholinesterase-functionalized acid purified multi-wall carbon nanotubes (CNTs) modified thick film strip electrode for organophosphorus (OP) insecticides was developed. The degree of inhibition of the enzyme acteylcholinesterase (AChE) by OP compounds was determined by measuring the electrooxidation current of the thiocholine generated by the AChE catalyzed hydrolysis of acteylthiocholine (ATCh). The large surface area and electro-catalytic activity of carbon nanotubes lowered the overpotential for thiocholine oxidation to 200 mV (vs. Ag/AgCl) without the use of mediating redox species and enzyme immobilization by physical adsorption. The biosensor detected as low as 0.5 nM (0.145 ppb) of the model organophosphate nerve agent paraoxon with good precision, electrode to electrode reproducibility and stability. Analysis of real water sample using the sensor demonstrated the feasibility of the application of the sensor for on site monitoring of OP compounds.

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Volume17, Issue1

January 2005

Pages 54-58

A Disposable Biosensor for Organophosphorus Nerve Agents Based on Carbon Nanotubes Modified Thick Film Strip Electrode

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A Disposable Biosensor for Organophosphorus Nerve Agents Based on Carbon Nanotubes Modified Thick Film Strip Electrode

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